Molecular Characterization and Expression of a Phytase Gene from the Thermophilic Fungus Thermomyces lanuginosus
نویسندگان
چکیده
منابع مشابه
Molecular characterization and expression of a phytase gene from the thermophilic fungus Thermomyces lanuginosus.
The phyA gene encoding an extracellular phytase from the thermophilic fungus Thermomyces lanuginosus was cloned and heterologously expressed, and the recombinant gene product was biochemically characterized. The phyA gene encodes a primary translation product (PhyA) of 475 amino acids (aa) which includes a putative signal peptide (23 aa) and propeptide (10 aa). The deduced amino acid sequence o...
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A chitinase gene from the thermophilic fungus, Thermomyces lanuginosus was amplified from the genomic DNA of the fungus, by PCR technique. The PCR product was cloned into pXcmkn12 vector by TA cloning technique and sequenced. The gene contained five introns and six exons. The deduced protein sequence coded for 390 amino acids and exhibited very high degree of similarity to other endochitinases ...
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 1998
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.64.11.4423-4427.1998